Size-controlled liposomes are essential for basic research and biotechnology. Current liposome homogenization methods lack precision, versatility, and/or scalability.
Dr. Chenxiang Lin’s lab at Yale invented a method to sort heterogeneous liposomes into a wide range of uniformly-sized populations by DNA-brick assisted density-gradient centrifugation.
Sorting is effective on premade liposomes with various sizes and contents. Protein and nucleic-acid cargos retain their functions after sorting.
The method is useful for the study of membrane biophysics and for formulation and prototyping of liposomal drug-carrying vehicles.
