Site-Specific Efficient Incorporation of Phosphoserine into Proteins Using a Novel EF-Tu and tRNA Charging System

• In general, phosphoproteins are highly unstable and difficult to produce.
• OCR3105/5254 pertains to the creation of a simple tool kit for the efficient site specific, phosphorylation signal-independent, introduction of phosphoserine into proteins in vitro and in vivo using a novel vector compatible with complementary bacterial strains and mammalian tissue culture.
• This technology provides a method of site specific cotranslational incorporation of phosphoserine into proteins, including human MEK-1.
• The production of phosphoprotein is inducible by phosphoserine and the system is compatible with transgenic methodologies.
• Applications:
  • research tools for the study of kinases and phosphatases
  • development of cell-based screens for new drug discovery
  • the manufacture of phosphoproteins for applications such as antibody generation
  • protein array manufacture
  • the target proteins in signal transduction pathways

• Issued Patent & Reference