Enrichment-free analysis of temporal dynamics of RNA

• Ability to monitor global steady state RNA turnover and distinguish acute transcriptional changes.
• Allows for the identification of isoform-specific transcript dynamics.
• Tags new transcripts with 4- thiouridine (s4U).
• 4-thiouridine is converted to into cytidine analogs which leads to U-C mutations and marks new transcripts upon sequencing.
• Broadly applicable to any application with metabolic labeling.